Department of Biochemistry and Molecular Biology, University of Rajshahi, Rajshahi-6205, Bangladesh.
GSC Advanced Research and Reviews, 2023, 16(03), 188–195.
Article DOI: 10.30574/gscarr.2023.16.3.0362
With the various side effects associated with synthetic medicines for treating diabetes, there is a need to develop herbal remedies as an alternative. Although Spinacia oleracea L. leaves are used to treat various health conditions, including diabetes, there is little research to validate its antidiabetic properties. This study aimed to investigate the hypoglycemic and hypolipidemic effect of methanolic and ethanolic extracts of Spinacia oleracea L. leaves against alloxan-induced hyperglycemia in mice. Hyperglycemia was induced by an injection of alloxan monohydrate 80mg/kg bw. (i.p.). After 72 hours, mice with Blood Glucose Levels above 11.0 mmol/L were selected for the investigation. Both methanolic and ethanolic extracts at 200 mg/kg bw. doses were observed to have antidiabetic effect for 21 consecutive days. Blood Glucose Level was monitored after 3, 6, 9, 12, 15, 18, and 21 days and compared with Glibenclamide (0.5 mg/kg b.wt.). Serum lipid profile [total cholesterol, triglycerides, low-density lipoprotein, and high-density lipoprotein] and liver enzymes such as SGPT, and SGOT were also determined. Oral administration of both extracts showed significant (P < 0.05) antihyperglycemic activity in alloxan-induced diabetic mice. The diabetic mice had significant (P < 0.01) reduction in blood glucose; serum liver enzyme level (SGPT and SGOT) and lipid profile were compared with normal mice.