In-vitro anti-inflammatory, anti-oxidant and In-vivo anti-urolithiatic activities of Coccinia grandis fruit extract

This research study was performed to evaluate the in-vitro anti-oxidant, anti-inflammatory and in-vivo activities of ethanolic fruit extract of Coccinia grandis. The fruit extract of Coccinia grandis at a concentration of 100 µg/ml was tested for its free radical scavenging activity adopting the standard methods like DPPH and Nitric Oxide. In-vitro anti-inflammatory activity was determined using Protein Denaturation Method and Human Red Blood Cells Method using Coccinia grandis. Further, In-vivo anti-urolithiatic activity was carried out by administering 0.75% of Ethylene Glycol solution to male rats for drinking, which caused urolithiasis. Coccinia grandis fruit extract exhibited significant anti-oxidant, anti-inflammatory and anti-urolithiatic activities. The results indicate that the extract possessed good therapeutic potential.